Solution：

Enzyme Concentration: Assuming that one molecule of salivary amylase hydrolyzes 5 mmol of starch molecules per second in the presence of sufficient substrate and all other conditions are appropriate, the rate of enzymatic reaction in this process is 5 mmol / s, The salivary amylase activity was also 5 mmol / s under this condition. If the number of enzyme molecules in an equivalent formulation is increased to 10, the enzyme concentration is increased by a factor of 10, then 50 mmol of starch molecules can be hydrolyzed in one second. The rate of enzymatic reaction during this process is 50 mmol / s.

Substrate concentration: the substrate in the lower concentration range, the higher the substrate concentration, the substrate and enzyme binding faster, so that the enzymatic reaction faster. Therefore, in the determination of enzyme activity, usually in the case of saturated substrate concentration was measured.

PH and temperature

Inhibitors and activators